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KMID : 0363320120330020197
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Journal of Korean Oriental Internal Medicine
2012 Volume.33 No. 2 p.197 ~ p.208
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Purification of Anti PC-3 Prostate Cancer Agents from Gleditsiae Spina
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Lim Se-Hyun
Lee Byung-Ho
Kim Young-Kyun
Cho Su-In
Kim Yong-Seong
Lim Chi-Yeon
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Abstract
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Objectives: Gleditsiae Spina has the effects of expelling toxins, draining pus, invigorating blood and resolving abscesses. Some clinicians apply the herb for patients suffering from cancer. However, its anti-cancer activities are not well understood. In the present study, anti-tumor agents from Gleditsiae Spina were purified.
Methods: The viability of the PC-3 cell line was determined using MTT assay, and the induction of apoptosis by Gleditsiae Spina extract in PC-3 cells was measured by Annexin-V/propidium iodide double staining assay detected by flow cytometry. TLC and HPLC analysis were used to separate and identify the anti-cancer agents.
Results: Treatment of the extract resulted in significant decreased cell viability of PC-3 cells in a dose- and time-dependent manner. Dose-dependent apoptotic cell death was also measured by flow cytometry analysis. The anti-cancer agents were successfully separated and identified by using TLC and HPLC analysis and the most potential agent among them was separated from EtOAC fraction.
Conclusions: These results might be applied in developing new drugs from natural resources like Korean traditional medicine, and also support the clinical usefulness of herbal medicine.
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KEYWORD
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Gleditsiae Spina, PC-3, apoptosis
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